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. 2007 Apr 26;404(Pt 1):23–29. doi: 10.1042/BJ20070146

Figure 3. Internalization of ephrinB2–Fc is necessary for cell re-spreading.

Figure 3

(A) Cells expressing K44A Dyn (green) do not internalize EphB–ephrinB2 complexes (red); acid-washing the cells immediately before fixation removed external ephrinB2–Fc, therefore red staining represents internalized ephrinB2–Fc. (B) EphrinB2-triggered retraction in cells injected with K44A Dyn, or with control injection marker (dextran). Results are means±S.E.M. (n=3–5, >30 cells/condition). (C) Time-lapse stills showing that K44A Dyn-injected cells do not re-spread lamellipodia after ephrinB2–Fc stimulation (see Supplemental Movie S2 at http://www.BiochemJ.org/bj/404/bj4040023add.htm). Scale bars, 20 μm (A), 30 μm (B).