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. 2007 Apr 26;404(Pt 1):89–96. doi: 10.1042/BJ20061404

Figure 5. HCa alters gating kinetics and stabilizes subconductances of RyR1 single-channel activity in BLMs.

Figure 5

RyR1 single channel was incorporated by inducing fusion of skeletal muscle junctional SR vesicles into BLMs. The channel activities were recorded and analysed as described in the Experimental section. RyR1 single channel in the absence of HCa was used to serve as control and was recorded for 3 min (A). Sequential additions of HCa to achieve a final concentration of 175 nM (B) and 350 nM (C) were made into the cis chamber, and the channel activity was recorded for 5 min under each condition. The broken lines indicate the maximum current amplitude of the native RyR1 channel (37.2 pA) when the channel is fully open (o). The arrow marked ‘c’ shows the zero current level when the channel is in the fully closed state, and the arrow marked ‘s’ shows the HCa-stabilized subconductance state of the channel. The data are representative of a total of five independent bilayer experiments with RyR1 channels from one junctional SR protein preparation and one purified RyR1 preparation.