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. 2007 Apr 26;404(Pt 1):131–140. doi: 10.1042/BJ20061747

Figure 1. ELISA of the binding of recombinant human NTN and ART to immobilized heparin.

Figure 1

Dose-dependence of (a) NTN and (b) ART binding. Competitive binding after pre-incubation with increasing concentrations of soluble heparin of 2 ng/well NTN (c) and 8 ng/well ART (d). The solid lines and closed symbols represent wells coated with heparin–BSA complex; the open symbols and dotted lines indicate control wells coated with mock-conjugated BSA. The results shown are means±S.E.M. for a single representative experiment, and each data point taken is from four or five replicate wells.