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. 2007 Apr 26;404(Pt 1):121–130. doi: 10.1042/BJ20061502

Figure 3. SDS/PAGE analysis of the purification of N-terminally His6-tagged residues 346–712 of Gfa1p overexpressed in E. coli.

Figure 3

Lane M, molecular-mass markers (in kDa); lane 1, total lysate of E. coli BL21(DE3)pLysS pET23b-FRU cells; lane 2, total lysate of control E. coli BL21(DE3)pLysS pET23b cells; lanes 3 and 4, wash fractions with buffer W; and, lane 5, fraction eluted by 0.5 M imidazole.