Table 1. PCR primers used for the construction of site-directed mutants of GFA1.

Restriction recognition sites are underlined. Capital letters indicate mismatches with the original GFA1 sequence (bold, introducing mutation; normal, introducing restriction recognition site).

Mutation	Primer name	Primer sequence
None	Gln1-KN	5′-cctggcggtacccatatgtgtggtatttttggttacg-3′
	cgfa-bam	5′-cacgggatccttactcaacagtaactgatttagcca-3′
W97G	Trpgly-3	5′-catgtgtGgCgcCtctagtatgagcaatacc-3′
	Trpgly-5	5′-catactagaGgcGcCacacatggtcaacc-3′
W97F	Trpphe-3	5′-agcGAaAcGCgtatgagcaataccaacatg-3′
	Trpphe-5	5′-ctcatacGCgTtTCgctacacatggtcaac-3′
V711F	Valphe	5′-cctgccctcgagttactcaaAagtaactgatttagccaag-3′