Table 3. Enzymatic activities of Gfa1p, its truncated and mutated versions.

The results are the means±S.D for three independent determinations.

	Amidohydrolysing activity (milliunits/mg)
Enzyme version	−Fru-6-P	+Fru-6-P	ISOM activity (units/mg)	GlcN-6-P-synthetic activity (units/mg)
Gfa1p (wild-type)	12±0.6	890±24	63.1±1.9	12±0.5
Δ218–283Gfa1p	11.6±0.2	12.5±0.7	61±2.2	<0.01
V711F	13±0.5	940±18	62.6±1.3	0.021±0.003
Δ709–712Gfa1p	290±6	357±11	60.7±1.4	<0.01
W97F	4.3±0.3	256±8	62.1±1.2	1.20±0.06
W97G	1.3±0.1	95±2	63.2±1.4	0.05±0.01