Fig. 3.

Ultraviolet B (UVB)-induced new synthesis of interleukin (IL)-8 by mast cells. (a) Partial inhibition of IL-8 synthesis by UVB-irradiated mast cells after treatment with various doses of cycloheximide (n = 3). (b) Expression of IL-8 mRNA and protein by mast cells 4 h after UVB-irradiation (n = 2). Histogram indicates real-time reverse transcription–polymerase chain reaction (RT–PCR) for IL-8 mRNA and line graph shows levels of IL-8 protein in supernatants of the same samples showing 10-fold increase in IL8 levels compared to control cells.