Fig. 6.

Methotrexate (MTX)-induced changes in intracellular apoptosis-related proteins. Peripheral blood mononuclear cells were grown for 7 days in the presence or absence of stimulating agents Candida albicans, tetanus toxoid or phytohaemaglutinin, with or without MTX for the last day. The cellular content of cleaved poly(ADP-ribose) polymerase, active caspase 3 and the anti-apoptotic protein Bcl-2 was subsequently measured by cytometric bead array. The results are shown as mean ± s.e.m. (n = 5). (*)P < 0·07, *P < 0·02, **P < 0·006 for no change induced by MTX.