Fig. 2.

Effect of acteoside treatment on histological parameters in acute colitis. Mice with established acute dextran sulphate sodium (DSS)-induced colitis received either acteoside (600 µg a day) or phosphate-buffered saline (PBS) (no acteoside) per mouse over 5 days. The epithelial damage (white bars), inflammatory infiltrate (grey bars) and total histological score (black bars, a) were determined as described in Materials and methods. The data presented are representative for four independent experiments with five mice per group. The boundary of the box indicates the 25th and the 75th percentiles; the line within the box marks the median. Whiskers indicates the 90th and 10th percentile. Whiskers including < 6 data points were not computed. *Significantly different from the total histological score of the no-acteoside group (a). Two representative colonic haematoxylin and eosin sections from animals treated with acteoside (b) or without acteoside (c) are shown (magnification 100×). From the marked areas, enlarged (three- to fourfold) areas are presented.