Fig. 2.

Antigen-specific induction of delayed-type hypersensitivity (DTH) arthritis. The mice were immunized intradermally with methylated bovine serum albumin (mBSA) (a, b) or KLH (c, d) (0·5 mg/body). Four days after the immunization, the mice were injected intravenously with anti-CII monoclonal antibody (MoAb) (0·5 mg/0·5 ml/body), and 7 days after the immunization the mice were challenged with mBSA (○) or key-hole limpet haemocyanin (KLH) (▴) into their right footpad (0·05 mg/0·05 ml/footpad). As a control, the other footpad was challenged by saline. As described in Materials and methods, the severity is represented by the clinical score and paw thickness. The data were expressed as the mean ± s.e.m. of the four mice in each group. The clinical score and the paw thickness of the mBSA- or KLH-challenged mice was significantly different from those of the KLH- or mBSA-challenged mice, respectively (*P < 0·05; **P < 0·01).