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. Author manuscript; available in PMC: 2007 May 16.
Published in final edited form as: Am J Physiol Cell Physiol. 2005 Jan 12;288(6):C1279–C1286. doi: 10.1152/ajpcell.00463.2004

Figure 7. 86Rb+-uptake by HKα2/NKβ1 complex but not by NKα1/NKβ1 complex is increased in CD63-knockdown cells.

Figure 7

Control HEK-293 cells (open bars) or CD63-knockdown HEK-293 cells (solid bars) were co-transfected with HKα2 plus NKβ1, NKα1 plus NKβ1, or pcDNA vector plus NKβ1. Ouabain (10 μ M) was used to block endogenous Na+,K+-ATPase of HEK-293 cells and 2 mM ouabain was used to block the transport activity of the transfected rat HKα2 or rat NKα1. Results (mean ± S.E.M.) represent the difference in 86Rb+-uptake when the experiments were performed with 10 μM ouabain vs. 2 mM ouabain. ** = 86Rb+-uptake in control HEK-293 cell is statistically different (P<0.01) from that in the CD63-knockdown cells for HKα2/NKβ1 -transfected cells. No statistical difference was observed between control and CD63-knockdown cells when the cells were co-transfected with NKα1/NKβ1 or pcDNA plus NKβ1. The experiment was repeated three times with similar results.