Table 1. Sense and antisense oligonucleotides used to amplify, by PCR, the carboxy-terminus of rat NKα₁ (r-NKα₁), rat NKα₂ (r-NKα₂), rat NKα₃ (r-NKα₃), rat HKα₁ (r-HKα₁), and rat or mouse HKα₂ (r/m-HKα₂).

For each sense oligonucleotide, the EcoRI (GAATTC) site used for cloning the insert in pGBKT7 is underlined. For each antisense oligonucleotide, the stop codon is marked with bold characters. The carboxy-terminus of each X⁺,K⁺-ATPase family member was defined by amino acid alignment using the PileUp program. The accession number of the DNA sequences used in these experiments are: r-HKα₁ = PO9626, r-NKα₁ = M14511, r-NKα₂ = M14212, r-NKα₃ = M14513, r-HKα₂ = M90398, m-HKα₂ = unpublished observations from our laboratory. The same oligonucleotide pairs were used to amplify the carboxy-terminus of rat and mouse HKα₂ (r/m-HKα₂.). Abbreviations: R = arginine, N = asparagine.

	Forward	Reverse	From-to (amino acid)	Length (amino acids)
r-NKα1	5′-ACGTGAATTCGTCTTCCAGCAGGGAATGAAGAAC-3′	5′-ACGTCTCGAGCTAGTAGTAGGTTTCCTTCTCCAC-3′	R941/Y1024	84
r-NKα2	5′-ACGTGAATTCGTGTTCCAGCAGGGCATGAAGAAC-3′	5′-ACGTCTCGAGTCAGTAGTACGTCTCCTTCTCCAC-3′	N939/Y1020	82
r-NKα3	5′-ACGTGAATTCGTCTTCCAGCAGGGCATGAAGAAT-3′	5′-ACGTCTCGAGTCAATAGTAGGTCTCTTTCTCCAC-3′	N932/Y1013	82
r-HKα1	5′-ACGTGAATTCGCTTTCCAGCAGGGATTCTTCAGG-3′	5′-ACGTCTCTCAATAGTAGAGTTCCTGGTCCCACCA-3′	N961/Y1033	73
r/m-Kα2	5′-ACGAGGAATTCCATCTTTCAGCAGGGGCTCTTCAG-3′	5′-ACGTTCTAGATTCAATAATACATGTTCTTATCCC-3′	N953/Y1036	84