Figure 2.

Amyloidogenic monomeric TTRs (M-TTRs) are not secreted efficiently from the cells. (A, B) Wild-type M-TTR, non-amyloidogenic M-TTRs (A) or amyloidogenic M-TTRs (B) were transiently transfected in CHO-K1 cells. Medium samples and the cell lysate samples were reduced and boiled before loading. Samples were analyzed by Western blotting using anti-human TTR or anti-calnexin (CNX) antibody. The arrowheads indicate the TTR monomer (M). ^*Indicates wild-type TTR protein purified from human serum.