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. 2007 Apr 19;26(10):2527–2539. doi: 10.1038/sj.emboj.7601689

Figure 4.

Figure 4

Quantification of autophagic vacuoles induced by ABT737. HeLa cells transfected with the indicated siRNAs (specific for Emerin or for Beclin-1 at 0 h) were re-transfected with LC3-GFP finally cultured in nutrient-free (NF) conditions (60–72 h), in the presence or absence 1 μM ABT737 and then subjected to electron microscopic detection of immature (AV1) or mature (AV2) autophagic vacuoles. Representative pictures are shown in (A). The number of AV1 and AV2 was determined for a minimum of 50 cells (means±s.e.m.) (B).