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. 2007 Apr 19;26(10):2477–2488. doi: 10.1038/sj.emboj.7601690

Figure 5.

Figure 5

Hst2 is required for rDNA and imr1 silencing in S. pombe (A) hst2Δ but not hst4Δ mutants derepress the rDNA locus. Fivefold dilutions of cells were plated onto EMM plates as a growth control (+ura), onto EMM plates lacking uracil (−ura) and onto 5-FOA plates (+FOA) to assay silencing of the ura4+ reporter gene inserted in the rDNA locus. Strains used were (wt) Hu393, (sir2Δ) Hu1408, (hst2Δ) Hu1409 and (hst4Δ) Hu1430. (B) hst2Δ derepresses the imr1 locus. Fivefold dilutions were plated onto EMM plates as a growth control (+ura), onto EMM plates lacking uracil (−ura) and onto 5-FOA plates (+FOA) to assay silencing of the ura4+ reporter gene inserted in the imr1 locus. Strains used were (wt) FY0498 and (hst2Δ) Hu1587. (C) A table summarizing the current and previous studies of Sirtuin mutant silencing phenotypes (−, defect; ±, partial or weak defect; +, no defect , that is, like wt).