Table 2.
Killing by colicin Ia requires Cir, but does not require other abundant outer membrane proteins
| Bacterial strain | Colicin Ia |
|---|---|
| BL21(DE3) (Cir and all porins present) | Sensitive |
| ASH102 (Cir-minus) | Resistant |
| MC4100 (Cir and all porins present) | Sensitive |
| RAM725 (MC4100, OmpF-minus [ompF::lacZ]) | Sensitive |
| RAM726 (MC4100, OmpC-minus [ompC::lacZ]) | Sensitive |
| RAM105 (MC4100, OmpF-minus, LamB-minus, OmpC-plus) | Sensitive |
| RAM123 (RAM105, OmpF-minus, LamB-minus, OmpC-plus, OmpG-plus) | Sensitive |
| B2045 (RAM105, OmpF-minus, LamB-minus, OmpC-minus, OmpG-plus) | Sensitive |
| RAM1129 (MC4100, TolC-minus [tolC::Kmr] | Sensitive |
| A586 (TolC point mutant) | Sensitivea |
| EW1b (TolC partial deletion) | Sensitivea |
| BL21(DE3) (Cir-minus)b | Resistant |
| BL21(DE3) (Cir-minus)+pET20b-cirA | Sensitive |
| BL21(DE3) (Cir M33C/V35P) | Resistant |
| Some tolC mutants are sensitive to colicin Ia but are killed less efficiently than wild type. | |
| The chromosomal cirA deletion in BL21(DE3) is described in Supplementary data. | |