Table 1.
Plasmids used in this studya
| Plasmidb | Relevant characteristics |
|---|---|
| virC1 and derivatives | |
| pKAB57 | pBSIIKS+ with PvirB-virC1 (XbaI site before stop codon) |
| pKAB58 | pBSIIKS+ with PvirB-virC1-gfp |
| pKAB110 | pBSIIKS+ with PvirB-virC1K15Q-gfp |
| pKAB187 | pBSIIKS+ with PvirB-virC1 |
| pKAB189 | pBSIIKS+ with PvirB-virC1K15Q |
| pKAB202 | pBSIIKS+ with PvirB-divIVA-virC1 |
| pKAB220 | pBSIIKS+ with PvirB-virC1K15E |
| pKA208 | pACYCDuet-1 with PT7-virC1 |
| pOB1 | pGEX-6P-1 with Ptac-GST-virC1 |
| pOB2 | pGEX-6P-1 with Ptac-GST-virC1K15Q |
| virC2 and derivatives | |
| pKA106 | pBSIIKS+ with PvirB-virC2 |
| pKAB113 | pBSIIKS+ with PvirB-virC2-gfp |
| pKA114 | pBBR1MCS2 with PvirB-virC2 |
| pKA115 | pBBR1MCS2 with PvirB-virC2-gfp |
| pKAB194 | pBSIIKS+ with PvirB-FLAG-virC2 |
| pSC1 | pET28b(+) with PT7-His,T7-virC2 |
| virC1,C2 and derivatives | |
| pKAB188 | pBSIIKS+ with PvirB-virC1,virC2 |
| pKAB190 | pBSIIKS+ with PvirB-virC1K15Q,virC2 |
| pKAB192 | pBSIIKS+ with PvirB-virC1,FLAG-virC2 |
| pKAB193 | pBSIIKS+ with PvirB-virC1K15Q,FLAG-virC2 |
| virD1 and derivatives | |
| pKA204 | pACYCDuet-1 with PT7-virD1 |
| pKVD16 | pKVD10 with Ptac-GST-virD1 |
| virD2 and derivatives | |
| pKA29 | pKVD10 with Ptac-GST-virD2 |
| pKAB195 | pBSIIKS+ with PvirB-FLAG-virD2 |
| pKA196 | pBBR1MCS2 with PvirB-FLAG-virD2 |
| pKA205 | pACYCDuet-1 with PT7-His-virD2 |
| virD4 derivatives | |
| pKA28 | pKVD10 with Ptac-GST-virD4Δ1-87 |
| pKA207 | pACYCDuet-1 with PT7-virD4Δ1-87 |
| see Supplementary Data and Supplementary Table S1 for construction details. | |
| Plasmids with ‘KAB' nomenclature were ligated to pBBR1MCS2 or pXZ153 BHR plasmids for introduction into A. tumefaciens. | |