Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Autoprocessing of rCPD is inhibited by NEM. rCPD (2 μg) was preincubated for 30 min with 1 mM NEM, E-64, Calpeptin, or PMSF, 100 μM Pepstatin, or 200 μM Leupeptin. Then, 5 μg of total cell protein was added to stimulate processing followed by overnight incubation. Proteins were separated by SDS–PAGE and stained with Coomassie R250. Arrows mark full-length (FL) and the processed (P) forms of rCPD.
