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rCPD processing is stimulated by guanine nucleotides. (A, B) rCPD (2 μg) was incubated alone (rCPD), with 5 μg of cytosolic protein preparation (Cytosol), or with 5 mM MgCl2 plus 5 mM of the indicated compound. (C) Incubation of 2 μg rCPD with the indicated concentration of GTPγS in the absence of MgCl2. All reactions were terminated after 2 h at 37°C. Proteins were separated by SDS–PAGE and stained with Coomassie R250. Arrows mark full-length (FL) and the processed (P) forms of rCPD.
