Figure 8.

rCPD is cleaved between L3428 and A3429. Processed rCPD eluted from C8 reverse phase column in two peaks at 10.56 and 10.88 min. The third peak at 12.31 min consists of poorly resolved materials such a polymers, small molecules, and peptides. The mass of the proteins contained within the peptide fragment peaks was determined by LTQ-FT analysis and the results are shown above the peak. Schematic representation of rCPD shows the determined cleavage site as well as the computer-predicted mass of the two fragments.