Table 2.
Identifications of differentially expressed spots in mDCs, moDCs and KG-1 cells using MALDI-TOF mass spectrometry
| Spot no. | Protein identification | Accession no. (Swiss-Prot) | Induction factor* (mDC/moDC) | Induction factor* (mDC/KG-1) | Theoretical pI/Mr | Sequence coverage (%) | MASCOT score | Biological process† |
|---|---|---|---|---|---|---|---|---|
| 1 | Fibrinogen γ chain | P02679 | 8.60 | 3.28 | 5.37/51,511.66 | 17 | 91 | 1,8,12,13 |
| 2 | Ubiquinol-cytochrome-c-reductase complex core protein 1 | P31930 | 24.41 | 21.01 | 5.94/52,618.79 | 13 | 58 | 1,2 |
| 3 | - | - | 8.05 | -2.27 | - | |||
| 4 | - | - | Not in mo | 21.06 | - | |||
| 5 | Glutathione S-transferase P | P09211 | 2.49 | 1.42 | 5.44/23,224.64 | 40 | 102 | 1, 2, 9 |
| 6 | L-lactate dehydrogenase B chain | P07195 | 1.70 | -1.28 | 5.72/36,507.30 | 33 | 175 | 2 |
| 7 | - | - | Not in m | Not in m | - | |||
| 8 | - | - | -4.06 | 9.51 | - | |||
| 9 | Pyridoxal kinase | O00764 | Not in m | Not in m | 5.75/35,102.30 | 22 | 96 | 2 |
| 10 | Nuclease isoform Sm3 | P13717 | 1.54 | 2.01 | 6.95/28,944.65 | 56 | 191 | 1, 2 |
| 11 | Annexin A1 | P04083 | 1.43 | 2.26 | 6.64/38,583.05 | 59 | 202 | 1, 4, 5, 7, 9 |
| 12 | Fructose-1,6-bisphosphatase | P09467 | -14.34 | 2.62 | 6.54/36,818.12 | 48 | 205 | 1, 2 |
| 13 | Fascin | Q16658 | -8.78 | -7.87 | 6.81/54,398.81 | 23 | 133 | 4,5,6 |
| 14 | Phosphoglycerate mutase I | P18669 | -1.37 | -2.21 | 6.75/28,672.74 | 47 | 143 | 1, 2 |
| 15 | Triosephosphate isomerase | P60174 | 1.02 | -1.14 | 6.51/26,538.30 | 56 | 209 | 2 |
| 16 | Actin related protein 2/3 complex subunit | O15144 | -16.74 | -20.03 | 6.84/34,333.02 | 19 | 91 | 4,5,6, 9 |
| 17 | Carbonic anhydrase II | P00918 | -15.63 | -10.99 | 6.86/29,114.86 | 29 | 88 | 1, 2 |
| 18 | Phosphoglucomutase I | Q16106 | -33.05 | -2.51 | 5.33/11,328.66 | 28 | 40 | 2 |
| 19 | Cystatin B | P04080 | -30.68 | -2.13 | 6.96/11,139.59 | 34 | 61 | 1, 2, 9 |
| 20 | - | - | -7.27 | -3.69 | - | |||
| 21 | - | - | -3.03 | -22.70 | - | |||
| 22 | Chain A, crystal structure of human Dj-1 | Q99497 | 1.85 | -2.02 | 6.33/19,891.05 | 36 | 89 | 1, 7 |
| 23 | Proteasome beta 2 subunit | Q9BWZ9 | -1.07 | -5.82 | 6.52/22,840.06 | 26 | 77 | 1,2,9 |
| 24 | dUTP pyrophosphatase isoform 2 | P33316 | Not in m or mo | Not in m or mo | 9.65/26,706.39 | 35 | 93 | 1,2, 9 |
MASCOT scores >64 were taken to be significant (p < 0.05). *Induction factor corresponds to the factor of difference between spot volume in mDCs compared with the respective mDC model. †Biological process: 1, cell growth/maintenance; 2, metabolism; 3, cell communication; 4, morphogenesis; 5, response to external stimulus; 6, cell motility; 7, response to stress; 8, circulation; 9, regulation of cellular processes; 10, cell differentiation; 11, death; 12, cell death; 13, coagulation; 14, homeostasis.