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. 2007 May 18;3(5):e77. doi: 10.1371/journal.pgen.0030077

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© 2007 Norais et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Sequence features of the second origin from main chromosome. Coordinates of the 9.3-kb NotI genomic fragment cloned in pTA416 and the 2.4-kb intergenic region cloned in pTA612 are shown. See Figure 1A for key. Numbering refers to TIGR contig number 455. Main chromosome, Chr.

(B) Sequence of repeats found at the intergenic region of oriC2 (numbered arrows in Figure 5A). The orientation is indicated by arrows and conserved sequences are shaded.

(C) Southern blot of PFG of DNA from strain H53, probed with intergenic region cloned in pTA612.