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. 2007 May 30;2(5):e477. doi: 10.1371/journal.pone.0000477

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Moll et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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RBC infected with the parasite clones/strains FCR3S1.2, 3D7AH1 and 7G8 were cultivated in the presence of α-Pf332-DBL/nDBL-antibodies for 24 h allowing reinvasion of the parasites into new RBC. The parasitemia was analysed by flow-cytometry and compared to a control cultivated in the presence of a serum raised against a non-related protein. Parasites displayed a decreased invasion rate in the presence of α-Pf332-DBL/nDBL-antibodies. The effect of α-Pf332-DBL/nDBL-antibodies (light bars) is slightly lower as compared to inhibition caused by antibodies against a hybrid protein of AMA1 and MSP1 (dark bars). Bars represent the mean of three experiments; error bars indicate the standard deviation.