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Western blot analysis of corneal tissue extracts with an anti-dermatopontin antibody: lane 1: molecular weight markers; lane 2: wild-type mouse cornea; lane 3: wild-type mouse lens; lane 4: bovine cornea; lane 5: recombinant human DPT (includes 10-His tag plus factor Xa cleavage site). The calculated molecular weights were 21,970 and 24,485 for the tissue-extracted and recombinant DPT, respectively.
