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. 2007 Mar 16;92(11):4109–4120. doi: 10.1529/biophysj.106.094649

FIGURE 1.

FIGURE 1

Three-dimensional single particle tracking. (A) Image set of an optical section of a QD at various focal positions. Upper panels are the fluorescence images of the QD at the various focal positions. Lower panels show the 3D expressions of the intensity profiles of the upper panels. (B) Relationship between the focal (Z) position and the fluorescence intensity. Black line is a single Gaussian curve fitted to the intensities. The peak occurred at 0.1 μm. (C) Spatial precision of 3D single particle tracking. Standard deviations of tracking using 3D single particle tracking were 6 nm (XY-plane) and 45 nm (Z axis). (D) Precision of the Z-direction determined using 3D single particle tracking. QDs bound on a coverslip were moved 100 nm along the focal axis by a Piezo actuator. The movement of the QDs was tracked using 3D single particle tracking in three dimensions. Only the Z-position of the QD is shown here.