FIGURE 5.

TBA does not affect TRPV1 single-channel conductance. (A, left) Single-channel recordings of TRPV1 activated by 4 μM capsaicin at 60 mV. The symbols O and C represent the open and closed current level, respectively. The right panel shows an all-points histogram (gray line) obtained from 30 traces as in the ones on the left; superimposed is the fit to a sum of two Gaussian functions (black line). The open level amplitude is 6 pA. (B) The patch in (A) was exposed to 250 μM TBA. The current traces were recorded also at 60 mV. Individual blocking events are discernible in the current trace as short-lived shut events. The all-points histogram from 25 traces is shown on the right with the fit to a sum of two Gaussians with open amplitude of 5.9 pA.