FIGURE 1.

Secondary structure of the M. jannaschii sR8 box C/D sRNA base paired with D and D′ target RNAs. M. jannaschii sR8 box C/D sRNA folded into its secondary structure to form the box C/D and C′/D′ motifs. Boxes C, C′, D, and D′ are indicated in bold. The box C/D and C′/D′ motifs form K-turn structures critical for the binding of archaeal box C/D sRNP core proteins with base-pairing of tandem-sheared G:A pairs indicated by dashed lines. D and D′ sRNA guide regions are base paired with the respective WT D and WT D′ target RNAs used in these studies. The boxed target nucleotide will be 2′-O-methylated during the sRNP-guided nucleotide modification reaction.