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The correlation of the prevalence of guanosine in the 51–63 base pair in 129 bacterial tRNA isoacceptors with the position of the tRNA on the EF-Tu binding hierarchy. Listed in parentheses on the horizontal axis is the EF-Tu binding affinity of the tRNA body as determined for E. coli tRNAs (Asahara and Uhlenbeck 2002). Green (G51-U63), yellow (U51-G63), red (C51-G63), and blue (G51-C63).
