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Histone acetylation in vivo in wild-type and mutant strains. Histones were extracted from yeast spheroplasts labeled with [3H]acetic acid. The acid extracts were then separated electrophoretically on acid-urea-Triton gels. (Left) Protein samples stained for total protein content by Coomassie brilliant blue staining. (Right) Fluorograph of the gel, after a 6-month exposure at −70°C. Strains tested were YCB496, 497 (mutant), and 180 (wild type).
