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Viral clearance responses induced by targeted DNA immunization were greater than those induced by nontargeted DNA immunization. Mice were immunized with 50 μg of plasmid DNA per leg in both quadriceps or with split virus as a control. After 65 days, the mice were challenged with a nonlethal dose of virus; 5 days later, the amount of infectious virus present in the lungs was determined by plaque assay. Titers for individual mice are shown.
