Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1975 Mar;29(3):422–426. doi: 10.1128/am.29.3.422-426.1975

Powdered Chitin Agar as a Selective Medium for Enumeration of Actinomycetes in Water and Soil1

S C Hsu *, J L Lockwood *
PMCID: PMC186990  PMID: 234719

Abstract

Agar media made with 0.4% colloidal chitin plus mineral salts and adjusted to pH 8.0 was superior to four other commonly used media for the isolation and enumeration of actinomycetes from water samples. More actinomycetes developed on chitin agar, and the development of bacteria and fungi was suppressed. Frozen and vacuum-dried chitin from aqueous colloidal suspensions was finely divided and gave results comparable to those obtained with media prepared from colloidal suspensions.

Full text

PDF
422

Images in this article

424Image
on p.424

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CORKE C. T., CHASE F. E. The selective enumeration of Actinomycetes in the presence of large numbers of fungi. Can J Microbiol. 1956 Feb;2(1):12–16. doi: 10.1139/m56-003. [DOI] [PubMed] [Google Scholar]
  2. CROOK P., CARPENTER C. C., KLENS P. F. The use of sodium propionate in isolating actinomycetes from soils. Science. 1950 Dec 1;112(2918):656–656. doi: 10.1126/science.112.2918.656-b. [DOI] [PubMed] [Google Scholar]
  3. EL-NAKEEB M. A., LECHEVALIER H. A. Selective isolation of aerobic Actinomycetes. Appl Microbiol. 1963 Mar;11:75–77. doi: 10.1128/am.11.2.75-77.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. JEUNIAUX C. Production d'exochitinase par des Streptomyces. C R Seances Soc Biol Fil. 1955 Jun;149(11-12):1307–1308. [PubMed] [Google Scholar]
  5. KUESTER E., WILLIAMS S. T. SELECTION OF MEDIA FOR ISOLATION OF STREPTOMYCETES. Nature. 1964 May 30;202:928–929. doi: 10.1038/202928a0. [DOI] [PubMed] [Google Scholar]
  6. Kuznetsov V. D., Iangulova I. V. Ispol'zovanie sredy s khitinom dlia vydeleniia i ucheta aktinomitsetov iz pochvy. Mikrobiologiia. 1970 Sep-Oct;39(5):902–906. [PubMed] [Google Scholar]
  7. LINGAPPA Y., LOCKWOOD J. L. A chitin medium for isolation, growth and maintenance of actinomycetes. Nature. 1961 Jan 14;189:158–159. doi: 10.1038/189158a0. [DOI] [PubMed] [Google Scholar]
  8. Lloyd A. B., Noveroske R. L., Lockwood J. L. Lysis of fungal mycelium by Streptomyces spp. and their chitinase systems. Phytopathology. 1965 Aug;55(8):871–875. [PubMed] [Google Scholar]
  9. PHILLIPS G. B., HANEL E., Jr Control of mold contaminants on solid media by the use of actidione. J Bacteriol. 1950 Jul;60(1):104–105. doi: 10.1128/jb.60.1.104-105.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. REYNOLDS D. M. Exocellular chitinase from a Streptomyces sp. J Gen Microbiol. 1954 Oct;11(2):150–159. doi: 10.1099/00221287-11-2-150. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES