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Belgrade rats have a glycine-to-arginine missense mutation in Nramp2. Multiple overlapping RT-PCR clones spanning the wild-type and b allele of Nramp2 were isolated and sequenced. A single polymorphism resulting in a glycine-to-arginine substitution at codon 185 was identified. The presence of the mutation, indicated by the arrow, was verified by sequencing genomic PCR clones of the region.
