Figure 4.
Tumor growth after combined treatment with radiation and AdK3. (A) Athymic mice with C6 glioma xenografts (165 ± 70 mm3) were treated with three locally fractioned doses of 7.5 Gy each and three intratumoral injections of 5 × 109 pfu of AdK3 or AdCO1 24 h after each irradiation. Treatment was initiated at day 10 (arrow) postgrafting (see Materials and Methods). Control animals were treated with three injections of AdK3 or AdCO1, or with three irradiations of 7.5 Gy each. Tumor volume was monitored until day 26 postgrafting (A), at which time they were extracted and analyzed (B). Representative tumors from the AdCO1-injected group (C Right), the AdCO1 + x-ray combination group (C Left), the AdK3-injected group (D Right), and the AdK3 + x-ray combination group (D Left) are shown at the same magnification. (E) Survival of AdK3-, x-ray-, or AdK3 + x-ray -treated mice 54 days after grafting. All mice were killed at day 54 for bioethical reasons.