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. 1998 Feb 3;95(3):1166–1171. doi: 10.1073/pnas.95.3.1166

Figure 2.

Figure 2

Immunoblot analysis of chimeric CFTR antibodies. (A) Purified CFTR 1 and CFTR 2 antibodies were analyzed by electrophoresis in a 10% nondenaturing polyacrylamide gel, blotted to nitrocellulose, and detected by anti-chicken IgG (H+L) (upper) and anti-mouse IgG (H+L) (lower). The marker on the right side indicates the predicted molecular component(s) of the band that are reacting with the detection reagent. Molecular mass standards are indicated in kilodaltons on the left. (B and C) The CFTR 1 and CFTR 2 antibodies, analyzed in A, were denatured in SDS/β-mercaptoethanol, separated by electrophoresis on a 12% polyacrylamide/SDS gel, blotted, and probed. (B) Region of the immunoblot containing the IgH chains: (upper) probed with anti-chicken IgG(H+L); (lower) probed with anti-mouse IgG (H+L). (C) Portion of the blots that contain the IgL chain, probed as described above.