Figure 3.

Phenotypic changes associated with antigen recognition on CD4⁺ TCR clonotype⁺ T cells after adoptive transfer into tumor-bearing mice. BALB/c mice were injected (i.v.) with 1 × 10⁶ A20WT or A20HA tumor cells. Nine days later all mice received 2.5 × 10⁶ anti-HA/I-E^d TCR⁺ transgenic T cells i.v. Fifteen days after transfer, T cells from non-tumor-bearing mice (patterned bars), mice bearing A20WT (hatched bars), or A20HA (solid bars) were isolated as in Fig. 2 and stained with Cy-Chrome-labeled anti-mouse CD4, biotinylated anti-TCR clonotype mAb 6.5 followed by PE-labeled streptavidin and FITC-conjugated anti-mouse CD44, anti-mouse CD45RB, or anti-mouse CD62L. Live gating on CD4⁺ T cells was set, and 100,000 events were collected per sample. Mean fluorescence intensity + SE is shown for each activation marker expressed by CD4⁺TCR clonotype⁺ T cells (four mice per group).