Table 1.

Response of T cells from vaccHA-primed mice

In vitro stimulation	[3H]Thymidine incorporation, cpm
	Normal mice	A20HA-bearing mice
None	3,026 ± 635	3,268 ± 1,203
HA peptide*	44,864 ± 9,168	11,155 ± 3,746
Vaccinia-infected splenocytes†	16,133 ± 2,307	17,359 ± 3,532
Con A‡	72,511 ± 10,204	59,115 ± 15,786
HA + IL-2§	64,114 ± 10,891	41,800 ± 4,177

BALB/c mice were given 1 × 10⁶ A20HA tumor cells intravenously or received no tumor. Nine days later, all mice received 2.5 × 10⁶ anti-HA/I-E^d TCR⁺ transgenic T cells. Nine days after T cell transfer, all mice were immunized with vacc-HA as in Fig. 4. On day +22 after T cell transfer, the mice were sacrificed and T cells were purified as before. Purified cells (4 × 10⁴ per well) were added to BALB/c splenocytes (8 × 10⁴ per well) and cultured with media alone or with the indicated stimulation. Con A stimulation was done without the addition of BALB/c splenocytes. [³H]Thymidine incorporation was determined after 3 days in culture. Values represent mean ± SE of triplicate cultures. 

^*HA peptide was 12.5 μg/ml. 

^†Normal BALB/c splenocytes were infected with vaccinia virus (3 pfu per cell) for 6 hr. Infected cells were washed three times and then cultured with purified T cells at different stimulator/responder ratios. Values represent proliferation at a ratio of 2:1. 

^‡Con A was 50 μg/ml. 

^§HA peptide at 12.5 μg/ml + IL-2 at 20 units/ml.