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SDS/PAGE and Western blot analyses of fractions collected during SE-HPLC of reaction mixture. Note the higher molecular weight Epo oligomers in earlier fractions. Epo trimers (∼117 kDa) and dimers (∼70 kDa) are well separated from Epo monomers (∼35 kDa). In this preparation, Epo tetramers were also detected in the earliest fractions.
