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. 2007 May 30;2(5):e488. doi: 10.1371/journal.pone.0000488

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Hehl et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Induced cells expressing Bax stop dividing and die in the course of 6 hours. Inset: semi-quantitative RT-PCR analysis of Bax mRNA levels in transgenic cells pre induction (PI) and 4 hours after induction. B) Subcellular localization of CWPs in ESVs of encysting wild type cells labeled by a monoclonal anti-CWP antibody at 4 h post induction. C) Bax abolishes ESV formation in encysting cells and causes accumulation of CWPs in the cytoplasm and the nucleoplasm. Inset: differential interference contrast (DIC) image. Scale bar: 5 µm.