Figure 2.
Analysis of inferior competitive long-term repopulating capacity of transplanted mpl−/− bone marrow cells. Southern blots of genomic DNA extracted from bone marrow cells from pairs of primary recipient mice that received 2 × 105 wild-type female competitor bone marrow cells mixed with 5 × 104, 2 × 105, or 2 × 106 test cells from male mpl−/− or wild-type (mpl+/+) mice (experiment 1). The filters were hybridized with the Y-chromosome specific pY2 probe, and the contribution of test cells to the bone marrow was determined in each case by comparison with a standard curve constructed from defined mixtures of male and female DNA. To correct the data for differences in the amounts of DNA loaded, male-specific DNA probes were stripped, and the membranes hybridized with a single copy c-myc DNA probe.