Table 2.

Cooperativity values

A–B	A + B	ΔGAB°, kcal/mol	ΔGA° + ΔGB°, kcal/mol	ΔGS°, kcal/mol	ΔHAB°, kcal/mol	ΔHA° + ΔHB°, kcal/mol	ΔHS°, kcal/mol	TΔSAB°, kcal/mol	TΔSA° + TΔSB°, kcal/mol	−TΔSS°, kcal/mol
Succinate	AcetateA + acetateB	−4.66 ± 0.07	−3.17 ± 0.09	+1.49 ± 0.11	−0.14 ± 0.04	+1.76 ± 0.18	+1.90 ± 0.18	+4.52 ± 0.08	+4.93 ± 0.20	−0.41 ± 0.22
Glutarate	AcetateA + acetateB	−4.28 ± 0.07	−3.17 ± 0.09	+1.11 ± 0.11	+0.73 ± 0.21	+1.76 ± 0.18	+1.03 ± 0.28	+5.01 ± 0.22	+4.93 ± 0.20	+0.08 ± 0.30
Tricarballate	SuccinateA + acetateB	−4.60 ± 0.07	−4.33 ± 0.09	+0.27 ± 0.11	−1.16 ± 0.33	+1.32 ± 0.30	+2.48 ± 0.45	+3.44 ± 0.34	+5.65 ± 0.32	−2.21 ± 0.47
Tricarballate	GlutarateA + acetateB	−4.60 ± 0.07	−4.30 ± 0.09	+0.30 ± 0.11	−1.16 ± 0.33	+1.92 ± 0.47	+3.08 ± 0.57	+3.44 ± 0.34	+6.22 ± 0.48	−2.78 ± 0.59
Butanetetra-carboxylate	SuccinateA + succinateB	−5.86 ± 0.07	−4.90 ± 0.10	+0.96 ± 0.12	−1.95 ± 0.56	+0.85 ± 0.29	+2.80 ± 0.63	+3.91 ± 0.56	+5.75 ± 0.31	−1.84 ± 0.64
Butanetetra-carboxylate	GlutarateA + glutarateB	−5.86 ± 0.07	−5.14 ± 0.09	+0.72 ± 0.11	−1.95 ± 0.56	+1.92 ± 0.47	+3.87 ± 0.73	+3.91 ± 0.56	+7.06 ± 0.48	−3.15 ± 0.74

Superscripts denote binding of carboxylate to A (host 2) or B (host 3). Thermodynamic measures of cooperativity are in bold.