Fig. 3.

PG fragments that accommodate two Tenebrio PGRP-SA molecules activate the prophenoloxidase system. (A Upper) The linearized PG only. (A Lower) Partially digested linearized PG by lysozyme was fractionated by a Toyopearl HW-55S column equilibrated with 20 mM Tris·HCl (pH 8.0) containing 150 mM NaCl. The phenoloxidase activity profile of each fraction is shown in red. (B Upper) A mixture of Tenebrio PGRP-SA with fraction 14 was injected onto the same column. (B Lower) A mixture of Tenebrio PGRP-SA and the fully digested PG that was fully digested with lysozyme by incubation for 16 h at 37°C was injected onto the same column.