Fig. 5.

Long and short RPTPβ both activate NF-κB responsive elements to similar levels. HEK 293 cells were transiently cotransfected with vector or with long-RPTPβ or short-RPTPβ cDNA constructs, along with the NF-κB luciferase reporter construct and a β-galactosidase transfection control. Twenty-four hours posttransfection, the cells were lysed and assayed for luciferase and β-galactosidase activity by using the Dual-Lite Reporter Kit (Applied Biosystems). Luciferase activity was normalized to β-galactosidase activity (error bars, ±SD; ** P < 0.005).