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Primary rat hippocampal neurons form active synapses on peptide scaffolds. The confocal images show bright discrete green labeling indicative of synaptically active membranes after FM1–43 incubation of neurons. (A) Active synapses on the peptide surface. (B) Active synapses on Matrigel. The active synapses on these different materials are not readily distinguishable, indicating that the peptide scaffold is a permissible substrate for synapse formation. Bar = 10 μm.
