Fig. 1.

Luciferase production in infected cells. 4C8 cells were infected with R3659 (control) or M016 luciferase-expressing Δγ₁34.5 HSV by using two different MOIs (0.1 and 1.0 PFU/cell), and supernates were harvested at 24, 48, or 72 h for determination of secreted luciferase by using a luminometer-based bioluminescence assay as described in the Materials and Methods section. Values shown represent the means of relative bioluminescence readings for three to four individual wells at each condition. Error bars represent standard errors of the mean.