Fig. 7.

Functional activity of GIMs: Allo-MLR. Stimulator cells were either GIMs isolated from patients’ tumors or APCs isolated from the peripheral blood of the respective glioma patients as described in Materials and Methods. Responder T cells were from an allogeneic normal donor, CFSE labeled, and incubated with the different stimulators. Following incubation, cells were labeled with PE-conjugated anti-CD3 antibody to further gate out the CFSE-labeled responder T cells for analysis by flow cytometry. The percentage of allo-T cells that underwent proliferation, as determined by dilution of the fluorescence intensity of the CFSE dye on gated CD3+ T cells, is indicated in each data plot. A. Controls included T cells incubated in media alone and T cells stimulated with anti-CD3 antibody. B. CFSE-labeled responder allo-T cells were incubated with the patient’s peripheral blood APCs either with or without LPS. C. CFSE-labeled allo-T cells were incubated with GIMs either with or without LPS.