Fig. 5.

Evidence that granule exocytosis occurs during the interaction between TALL-104 and glioma cells. A. Calcein-loaded 04-11-MG glioma cells before (upper panel) and then at 5000 s after the addition of TALL-104 cells (lower panel). The arrows in each of the panels point to the locations of adherent fluorescent cells initially present that lost fluorescence during the experiment. B. Fluorescence traces from individual cells shown in A. The upper trace is an example of a cell that did not release calcein; the second, third, and bottom three panels monitor individual cells that displayed calcein release at 900 s, 1500 s, and 3100 s, respectively, after the addition of the TALL-104 cells. C. Time course of hitting for the experiment shown in A and B. Approximately 25% of cells were hit over the 5000-s (83-min) duration of the experiment.