Table 3.
Decrease in mitosis of glioma cells and increase in apoptosis upon coincubation with TALL-104 cells as determined by an in vitro morphologic assaya
| % Mitotic | % Apoptotic | |||||
|---|---|---|---|---|---|---|
| Tumor ± TALL-104 | 1h | 2h | 4h | 1h | 2h | 4h |
| 04-11-MG | 1 | 1 | 10.7 | 6.6 | 5.5 | 2.7 |
| 04-11-MG + TALL | 0 | 0 | 3.4 | 51.9 | 45.9 | 29.1 |
| 10-08-MG | 10.4 | 1.6 | ||||
| 10-08-MG + TALL | 2.7 | 55.7 | ||||
| 14-07-MG | 4.1 | 3.2 | ||||
| 14-07-MG + TALL | 1.3 | 36.9 | ||||
a
Values obtained from microscopic counts of H&E-stained glioma cells attached to 4-well chamber tissue culture slides. Apoptotic and mitotic figures were determined by light microscopy at 1, 2, or 4 h after coincubation of TALL-104 cells with glioma cells. Eight hundred cells were examined and scored as normal, mitotic, or apoptotic. The E:T ratio was 10:1.