Table 4.
Cytokines are secreted when irradiated TALL-104 cells are coincubated with brain tumor cells, but secretion is decreased in the presence of dexamethasone (units in pg/ml)
| Coincubated agentsa | IFN-γ | GM-CSF | TNF-α | TNF-β |
|---|---|---|---|---|
| DBTRG-05MG + TALL | 4.8 | 68.4 | 45 | 21.8 |
| DBTRG-05MG + TALL + Dex | 0 | 14.2 | 0 | 0 |
| 04-11-MG + TALL | 0 | 19.4 | 45 | 0 |
| 04-11-MG + TALL + Dex | 0 | 2.8 | 6.6 | 5 |
| 10-08-MG + TALL | 16 | 93.4 | 0 | 33.6 |
| 10-08-MG + TALL + Dex | 0 | 10.2 | 0 | 0 |
a
DBTRG-05MG, 04-11-MG, and 10-08-MG are all derived from glioblastoma cell explants at passages 12, 18, and 8, respectively. Human brain tumor cells were cocultured with irradiated TALL-104 cells for 18 h. Supernates were harvested, clarified, and tested for the presence of cytokines by enzyme-linked immunosorbent assay. Cytokines were not detected in the supernates from brain tumor cells or TALL-104 cells cultured alone.