Table 6.

Phenotypic expression of FasL by TALL-104 cells and Fas by glioma cells

Cells assayeda	Coincubated cells	Antibody	% cells positive	MFIb
TALL-104	None	Anti FasL	1.23	0.26
TALL-104	10-08-MG	Anti FasL	0.1	1.0
TALL-104	04-11-MG	Anti FasL	2.2	3.6
TALL-104	14-07-MG	Anti FasL	0.1	1.0
10-08-MG	none	Anti Fas	100	7.83
04-11-MG	none	Anti Fas	99.8	6.21
14-07-MG	none	Anti Fas	99.8	5.52

Abbreviations: FasL, Fas ligand; MG, malignant glioma.

^aThe cell type assayed was either incubated alone or coincubated with carboxyfluorescein diacetate succinimidyl ester–labeled MG cells for 16 h prior to assessing for upregulation of FasL on the TALL-104 cells by flow cytometry.

^bMFI is mean fluorescence intensity reflective of the relative antigen density.