Table 2.
Relative expression of Giardia intestinalis core histone genes
| Gene | Accession #1 | PCR primers (5' → 3')2 | Avg. PCR efficiency3 | Avg. Ct4 |
| H4 | AF139876 | F: AGACCCGCGAGGTCCTCAA | 91 ± 9% | 15.9 ± 0.8 |
| R: TTGCGCTGGCCGTGTTCTG | ||||
| H3 | AF139875 | F: TACCAGAAGTCCACAGACC | 95 ± 10% | 16.0 ± 0.6 |
| R: TGGAAGCGGATGTCGGA | ||||
| H2a | AF139873 | F: GTCGTGGCAGAGGTCTT | 102 ± 15% | 16.9 ± 1.2 |
| R: CTCCTTGTCCTTGCGGA | ||||
| H2b | AF139874 | F: GACAACATCCGCTCCGA | 101 ± 16% | 17.8 ± 1.8 |
| R: CGAAGAGGTCGTTCACG | ||||
1 Accession number for nucleotide sequence in GenBank
2 The sequences are denoted F for the forward primer, and R for the reverse primer.
3 Average PCR amplification efficiencies and standard deviations are calculated from triplicate reactions performed in 3 to 5 independent experiments.
4 Average Ct values and standard deviations are calculated from triplicate reactions performed in 3 to 7 independent experiments.